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Coppa Italia
But then a Cup match is always different, and Roma manager Rudi Garcia is confident his side can overcome that heavy loss and advance to the semifinals in front of their own fans.
"Our aim is to reach the semifinal," Garcia said on Monday. "We're not thinking about the league and our sole focus is on winning tomorrow.
"It's a one-off encounter and we have one sole aim: reaching the semifinal. If we want to get there, we'll need to put in a great performance."
Garcia has asked the home fans to get behind their team on Tuesday night and act as the proverbial 12th man when Juventus pay a visit.
"We'll be in front of a full house at the Olimpico and it will be a terrific atmosphere, comparable to the one we faced at Juventus Stadium, where it's easy to play with the support of the crowd," Garcia added. "The fans will be fired up and will get behind the team.
"We responded well to our defeat in Turin. It was important to win and we did just that against Sampdoria. The fixture list throws up bizarre occurrences and sees us back up against Juventus straightaway. I've got no doubts over my team, they give their all in every game."
Sampdoria was the team that Juventus beat at the weekend, for their 12th consecutive victory, with Fernando Llorente, Arturo Vidal and Paul Pogba scoring in a 4-2 win. Llorente urged his side to keep the foot on the pedal to ensure the winning streak doesn't end at 12.
"Our team is doing really well as we are -- we need to continue along this path. The important thing is winning, and we're doing that well," Llorente told the club's official website.
"It was important to win today [Saturday] and we've done it. Sampdoria proved difficult opposition and played a great game. They caused us some problems, and showed the strength they have in their ranks.
"Now it's time for the Coppa Italia. We'll be playing against very strong opposition in Rome."
Juventus playmaker extraordinaire Andrea Pirlo is expected to come back into the starting lineup after missing out against Sampdoria, but Garcia stressed on the importance of concentrating on their own game, rather than worrying too much about Juve's strengths.
"We can't just think about handling Pirlo, the danger can arrive from across the park," the Frenchman said. "But it's the same for Juventus, we've had many players on the scoresheet this season.
"Pirlo is a world-class player and if he has a quiet day then all the better for us. But we need to stick to our game plan and be clinical and efficient. I hope we've saved a few goals for this one."